HIV-1 CCR5 tropism prediction
Description:
In house assay that genotypes the envelope V3 loop of HIV-1 gp120.
Clinical details:
HIV infects cells expressing the primary receptor CD4 and one of the chemokine receptors CCR5 or CXCR4. CCR5 antagonists, such as the anti-retroviral drug Maraviroc, inhibit replication of R5 virus. Tropism testing is essential prior to use of this drug in order to indentify individuals harbouring X4 or R5/X4 variants of HIV in whom no significant virological response to Maraviroc treatment would be anticipated.
Reference range:
Not applicable. Result given as predicted tropism
Synonyms or keywords:
HIV, Tropism, genotyping, Maraviroc, sequencing
Units:
Predicted co-receptor use categorised as;
CCR5 (R5),
CXCR4 (X4),
dual tropic (R5/X4) or
mixed tropic (R5,X4,R5/X4).
Department:
Location:
Sample type and Volume required:
10 ml EDTA plasma, CSF
Call in advance:
For samples with a viral viral load <1,000 copies/ml
Turnaround time:
2 weeks
Storage and transport:
Room temperature or refrigerated if there is a delay in transport
Contacts:
South London Specialist Virology Centre
Results: 02032996155 Medical Advice: 02032996298
King's College Hospital
Cheyne Wing, 2nd Floor (opposite Liver ITU)
Denmark Hill
London SE5 9RS
DX address: South London (PHL) Kings DX 6570200 Peckham 90SE
Cheyne Wing, 2nd Floor (opposite Liver ITU)
Denmark Hill
London SE5 9RS
DX address: South London (PHL) Kings DX 6570200 Peckham 90SE
Laboratory:
Last updated: 07/08/2015
