Emicizumab activity

Description: 
Emicizumab (Hemlibra) is a bispecific antibody which acts as a coagulation Factor VIII mimetic. Circulating emicizumab is measured using a modified one-stage clotting assay calibrated with emicizumab reference standard.

This test is not currently included in the laboratory's scope of UKAS accreditation to ISO15189:2012.
Clinical details: 
Emicizumab has a longer half life in the circulation than convential FVIII replacement therapy. The use of standard coagulation assays for assessing emicizumab is not recommended due to an oversensitivity of its effect on standard assay results.
Related condition or disease: 
Haemophilia A
Reference range: 

40 – 60 µg/mL

Synonyms or keywords: 
Hemlibra, EHL
Units: 
µg/mL
Department: 
Haemostasis and Thrombosis Department
Location: 
St Thomas' Hospital
Sample type and Volume required: 
External requests: Citrated platelet poor plasma (500µL x 1 aliquot) or 3.5mL whole blood citrate Internal requests: please refer to EPR label
Turnaround time: 
7-12 days
Special sample instructions: 

The sample should be analysed or processed & stored within 4 hours of venepuncture. Please ensure sample tubes are filled exactly to the fill-line as underfilling or overfilling creates a dilution error and leads to inaccurate results.

Criteria for acceptance / rejections of sample
Storage and transport: 
It is advised that citrated plasma is frozen prior to transport and sent to the laboratory on dry ice to maintain sample quality and integrity. Whole blood can be sent at room temperature, but must be received within 4 hours of venepuncture. Suggest laboratory contacted in advance of sample dispatch.
Contacts:
Diagnostic Haemostasis and Thrombosis Department
St Thomas': 020 7188 2797; Guy's: 020 7188 7188 ext. 53860
St Thomas' Hospital
North Wing - 4th and 5th Floors
Westminster Bridge Road
London SE1 7EH

Laboratory opening times
24/7

Guy's Hospital
Southwark Wing - 4th Floor
Great Maze Pond
London SE1 9RT

Outside core hours, contact Duty Haemostasis Biomedical Scientist
For clinical advice or interpretation of results, please contact the laboratory in the first instance.
Laboratory: 
Diagnostic Haemostasis and Thrombosis Laboratory at Guy's and St Thomas'

Print as a PDF

Last updated: 22/10/2021