ADA (adenosine deaminase) - Enzyme Assay
Description:
Adenosine deaminase (ADA) catalyses the deamination of adenosine and deoxyadenosine to inosine and deoxyinosine respectively. A deficiency of ADA leads to the accumulation of toxic dATP in T- and B- cells. The enzyme is assayed in red cell lysates as the conversion of adenosine to inosine using an HPLC-based method.
This test is not currently included in the laboratory's UKAS scope of accreditation to ISO15189:2012.
This test is not currently included in the laboratory's UKAS scope of accreditation to ISO15189:2012.
Clinical details:
Adenosine deaminase (ADA) deficiency is a cause of severe combined immunodeficiency. Patients typically present in the first few weeks of life with recurrent infections and failure to thrive. There is a lack T and B cell immunity and absent lymph nodes.
High ADA enzyme activity levels are found in cases of Diamond-Blackfan anaemia (DBA), a congenital erythroid aplasia that usually presents in infancy.
High ADA enzyme activity levels are found in cases of Diamond-Blackfan anaemia (DBA), a congenital erythroid aplasia that usually presents in infancy.
Reference range:
40 - 100
Units:
nmol/h/ mgHb
Department:
Location:
Last updated: 20/02/2023
