Fibrinogen antigen

Description: 
Immuno-turbidimetric assay using latex particles coated with polyclonal rabbit anti-human fibrinogen antibodies, for the in vitro determination of fibrinogen. When the tested sample is mixed with the latex reagent (R1), the anti-fibrinogen antibodies coupled onto latex particles react with fibrinogen present in the sample and agglutination occurs. The amount of agglutination is directly proportional to the amount of fibrinogen in the sample and is measured by light absorption (at a wavelength of 405nm).

This test is not currently included in the laboratory's UKAS scope of accreditation to ISO15189:2012.
Clinical details: 
The final stage in the molecular co-operation of the procoagulant participants of secondary haemostasis is the conversion of soluble fibrinogen monomers to insoluble, cross-linked fibrin polymers to stabilse the blood clot. Fibrinogen is a large, symmetrical dimeric glycoprotein composed of two identical sub-units, each of which is comprised of three non-identical polypeptide chains. It is present in plasma at a high concentration and is also contained in platelet α-granules. Fibrin is formed by thrombin cleavage of the small fibrinopeptides A & B from intact fibrinogen molecules that exposes donor sites that interlock with complementary unshielded acceptor sites on adjacent molecules to promote polymerisation.Quantitative and qualitative deficiencies of fibrinogen can be congenital or acquired and give rise to bleeding. More rarely, some dysfibrinogenemias can predispose to thrombosis.
Related condition or disease: 
Bleeding disorder
Reference range: 

1.74-3.48g/L

Units: 
g/l
Department: 
Haemostasis and Thrombosis Department
Location: 
St Thomas' Hospital
Sample type and Volume required: 
External requests: Citrated platelet poor plasma 500µL x 1 aliquot.
Internal requests: please refer to EPR label
Turnaround time: 
7 - 14 days
Special sample instructions: 

The sample should be analysed or manipulated & stored in the laboratory within 4 hours of venepuncture. Please ensure sample tubes are filled exactly to the fill-line as underfilling or overfilling creates a dilution error and leads to inaccurate results.

Criteria for acceptance / rejections of sample
Storage and transport: 
It is advised that citrated plasma is frozen prior to transport and sent to the laboratory on dry ice to maintain sample quality and integrity.
Contacts:
Diagnostic Haemostasis and Thrombosis Department
St Thomas': 020 7188 2797; Guy's: 020 7188 7188 ext. 53860
St Thomas' Hospital
North Wing - 4th and 5th Floors
Westminster Bridge Road
London SE1 7EH

Laboratory opening times
24/7

Guy's Hospital
Southwark Wing - 4th Floor
Great Maze Pond
London SE1 9RT

Outside core hours, contact Duty Haemostasis Biomedical Scientist
For clinical advice or interpretation of results, please contact the laboratory in the first instance.
Laboratory: 
Diagnostic Haemostasis and Thrombosis Laboratory at Guy's and St Thomas'

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Last updated: 09/07/2021