HLA Class I and II typing for Disease Association

Description: 
HLA typing can assist in resolving differential diagnosis of HLA associated disorders. A variety of methods and technologies are used, including Next Generation Sequencing (NGS), PCR-SSP and PCR-SSOP (Luminex) to determine HLA Class I (HLA-A, B, C) and/or HLA Class II (DRB1/3/4/5, DQB, DQA, DPB, DPA) alleles. The method of testing and the locus of HLA tested will depend on the disease under investigation and the clinical details provided.
Clinical details: 
The associations between HLA genes with a variety of autoimmune and inflammatory diseases are well established, even where the actual disease susceptibility genes or the mechanism that confers the disease often remain to be elucidated. Knowledge of a patients HLA type can assist in confirming or discounting a diagnosis. The list provided below is not exhaustive – please contact the laboratory to discuss any additional disease association queries.
Related condition or disease: 
Birdshot Retinopathy – HLA-A*29; Behcet’s Disease – HLA-B*51; Psoriasis – HLA-C*06; Dermatitis Herpetiformis – HLA-A*01, B*08, DRB1*03; Linear IgA disease – HLA-B*08, DRB1*03; Coeliac Disease – HLA-DQB1*02/DQA1*05, DRB1*03:02/DQA1*03:01 heterodimers, Actinic Prurigo – HLA-DRB1*04:07; Pemphigus Vulgaris – HLA-DRB1*04:02, HLA-DRB1*14:01/54; Narcolepsy – HLA-DQB1*06:02; Vulvovaginal Gingivitis – HLA-DQB1*02:01; Epidermolysis Bullosa Acquisita – HLA-DRB1*15:03; Lichen Planus – HLA-DRB1*03; Mucous Membrane Pemphigoid – DQB1*03:01; Pemphigus Foliaceus – DRB1*01, DRB1*16.
Reference range: 

none

Synonyms or keywords: 
HLA typing, Disease Association
Units: 
none
Department: 
Clinical Transplantation Department
Location: 
Guy's Hospital
Sample type and Volume required: 
10ml EDTA blood
Call in advance: 
No
Turnaround time: 
10 working days
Special sample instructions: 

none

Criteria for acceptance / rejections of sample
Storage and transport: 
UN3373 packaging, room temperature
Time limit for extra tests: 
none
Contacts:
Clinical Transplantation Department at Guy's Hospital
Laboratory Enquiry: 020 7188 1540
Clinical Transplantation Department
Guy's Hospital
Borough Wing - 3rd Floor
Great Maze Pond
London SE1 9RT
For clinical advice or interpretation of results, please contact the laboratory in the first instance.
Laboratory: 
Clinical Transplantation Laboratory at Guy's Hospital

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Last updated: 11/08/2023