HLA antibody titre crossmatch by flow cytometry

Description: 
Flow cytometry analysing IgG binding to recipient T and B lymphocytes from peripheral blood
Clinical details: 
This test is used to gauge the amount of donor specific antibody present. It allows assessment of whether the antibody may be removed to allow transplantation. If antibody removal is feasible recipient serum samples pre and post a 3litre plasma-exchange procedure will allow assessment of the number of Pex required for removal to allow transplantation.
Related condition or disease: 
HLA antibody incompatible transplantation
Reference range: 

> 2.3 - positive

Synonyms or keywords: 
crossmatch incompatible HLAi
Units: 
RMF
Department: 
Clinical Transplantation Department
Location: 
Guy's Hospital
Sample type and Volume required: 
donor - 20mls EDTA blood
recipient - 5ml clotted blood
Call in advance: 
Call 020 7188 1540 to book in a crossmatch
Turnaround time: 
2 days
Special sample instructions: 

samples should be bled on day of testing

Criteria for acceptance / rejections of sample
Storage and transport: 
UN3373 packaging
Time limit for extra tests: 
none
Contacts:
Clinical Transplantation Department at Guy's Hospital
Laboratory Enquiry: 020 7188 1540
Clinical Transplantation Department
Guy's Hospital
Borough Wing - 3rd Floor
Great Maze Pond
London SE1 9RT
For clinical advice or interpretation of results, please contact the laboratory in the first instance.
Laboratory: 
Clinical Transplantation Laboratory at Guy's Hospital

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Last updated: 07/08/2015